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Fig. 4. Western blot of proteins from cell bodies of wild-type and tua2-2R1 cells. Increasing amounts of protein were loaded to detect the predicted 39 kD truncated protein of tua2-2R1 cells. Lane 1: 7 µg of protein from wild-type cells. Lanes 2-5: 7 µg, 15 µg, 30 µg and 50 µg of protein from tua2-2R1 cells. Truncated tubulin protein is not detectable in tua2-2R1 extracts, only wild-type tubulin.





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