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Fig. 3. Determination of the number of parasites present in dendritic cells (DCs) infected with various Leishmania developmental stages opsonized or not with specific Abs. DCs were incubated with either amastigotes from nude mice (Am nude) covered or not with Abs, or stationary phase promastigotes (SPm) or with metacyclic promastigotes (MPm Ficoll) previously coated or not with Abs. Twenty-one hours (white bars) or 69 hours (grey bars) after adding parasites, DCs were recovered, fixed, permeabilised and stained as in Fig. 2. Intracellular parasites were detected by fluorescence microscopy. For each determination, the number of parasites present in at least 100 infected DCs were counted.
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