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Fig. 7. Kinetics of CD86 cell surface expression in the uninfected and Leishmania-infected dendritic cells (DCs) present in the same cell populations. On day 14, DCs were cultured for an additional 21 hours (upper panels) or 69 hours (lower panels) (i) in the absence of parasites (no parasites) or with (ii) BCG or (iii) parasites devoid of Abs (Am nude, MPm Ficoll), or (iv) Ab-coated parasites (Am nude + IS, MPm Ficoll + IS). DCs were double labelled with a CD86-specific mAb (FL2) and the 2A3-26 mAb (FL1). In each dot plot, the cells expressing high levels of CD86 molecules were subdivided into FL1 low (left region corresponding to uninfected DCs) and FL1 high (right region corresponding to infected DCs) DCs. The values indicated in the left and right regions are the percentages of CD86^high DCs related to uninfected and Leishmania-containing DC sub-populations, respectively. The results shown are representative of three similar experiments.

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