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Fig. 3. Phosphorylation of STAT3 in nuclear bodies. HepG2 cells transiently transfected with STAT3-YFP were stimulated with IL-6 (20 ng/ml) for 30 minutes or left unstimulated as indicated. Subsequently, cells were fixed and incubated with (A) STAT3 phosphotyrosine-specific antibodies or (B) STAT3 phosphoserine-specific antibodies. A TRITC-conjugated secondary antibody was used for immunostaining. Cells were analyzed by confocal microscopy. Bars, 10 µm.





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