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Fig. 5. Localization of SC-35, GFP-SF2/ASF, PML and STAT3. (A) HepG2 cells were transfected with STAT3-YFP. Cells were stimulated with IL-6 for 30 minutes or left unstimulated as indictated. Subsequently, cells were fixed and SC-35 was immunostained with a SC-35-specific antibody and a TRITC-conjugated secondary antibody. Images were taken by confocal microscopy. Bars, 10 µm. (B) HepG2 cells were cotransfected with the splicing factor fusion protein GFP-SF2/ASF and STAT3. After IL-6 treatment for 15 minutes cells were fixed and STAT3 was immunostained with a STAT3-specific antibody and a TRITC-conjugated secondary antibody. Images were taken by confocal microscopy. Bars, 10 µm. (C) HepG2 cells were transfected with STAT3-YFP. Cells were stimulated with IL-6 for 30 minutes or left unstimulated. Subsequently, cells were fixed and PML was immunostained with a PML-specific antibody and a TRITC-conjugated secondary antibody. Images were taken by confocal microscopy. Bars, 10 µm.





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