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Fig. 6. Localization of acetylated histone H4, CBP and STAT3. HepG2 cells were transfected with STAT3-YFP. Cells were stimulated with 20 ng/ml IL-6 for 30 minutes or left unstimulated. Subsequently, cells were fixed and (A) CBP or (B) acetylated histone H4 was immunostained using the respective specific antibodies and a TRITC-conjugated secondary antibody. Images were taken by confocal microscopy. White circles mark selected STAT3 bodies that colocalize with domains of CBP or acetylated histone H4, respectively. Bars, 10 µm.





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