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Fig. 7. Localization of nascent RNA and STAT3 nuclear bodies. HepG2 cells were transfected with STAT3-YFP. Fortyeight hours after transfection cells were pre-incubated in medium supplemented with 1 mM bromouridine for 30 minutes. Subsequently, cells were stimulated with 20 ng/ml IL-6 for another 30 minutes in the presence of bromouridine. Images of fixed cells were taken by confocal laser-scanning microscopy (upper panel). Intensities of the BrU and STAT3-YFP signals are also shown in false-color mode, indicating highest intensities in white and red and lowest intensities in blue and black (lower panel). White circles mark a selected area where high levels of STAT3 and nascent RNA were detected. Bars, 10 µm.





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