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Fig. 8. Actinomycin D has no effect on STAT3-YFP nuclear body formation. HepG2 cells were transfected with STAT3-YFP, preincubated with 4 µM actinomycin D in DMSO for 2 hours or left without inhibitor and were subsequently treated for 30 minutes with 20 ng/ml IL-6 or left untreated as indicated in the figure. Images of fixed cells were taken by confocal laser-scanning microscopy. DMSO treatment alone was taken as a control. Bars, 10 µm.





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