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Fig. 2. {alpha}1,6-Mannosylation of bulk glycoproteins in the ER. Sec13-1 (H230), sec23-1 (H481) and {Delta}och1 (H1691) cells were preincubated for 15 minutes and 35S-labeled for 30 minutes at 37°C. The lysed cells were precipitated with concanavalin A-Sepharose. Half of the precipitates were subjected to SDS-PAGE directly (uneven lanes). The proteins of the other half of the samples were released from the lectin beads and immunoprecipitated with {alpha}1,6-mannose antiserum (even lanes).





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