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Fig. 4. Electrophoretic migration of Hsp150{Delta}-ß-lactamase-HDEL. Normal cells expressing Hsp150{Delta}-ß-lactamase-HDEL (H606; lanes 1 and 2) or Hsp150{Delta}-ß-lactamase (H335; lanes 3 and 4), and a sec18-1 mutant expressing Hsp150{Delta}-ß-lactamase-HDEL (H610; lanes 5 and 6) were preincubated for 15 minutes and 35S-labeled for 30 minutes at 37°C. The medium (m) and respective cell lysate (c) samples were immunoprecipitated with ß-lactamase antiserum and analysed by SDS-PAGE. The figures on the right indicate biosynthetic intermediates of the reporter proteins and those on the left molecular weight markers.





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