spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 5. Scw4p glycosylation in a COPI mutant. Control (H1495), sec23-1 (H1496) and sec21-1 (H1497) cells were preincubated at 37°C for 15 minutes, and pulse-labelled with [35S]methionine/cysteine for 5 minutes. Parallel samples were chased with CHX for 30 minutes, as indicated. TM was present from the preincubation onwards, as indicated. The cell lysates were immunoprecipitated with antibody against pentahistidine only (lanes 1-12), or reimmunoprecipitated with antiserum against {alpha}1,6-mannose (lanes 13-15), followed by SDS-PAGE analysis. Apparent molecular weights of Scw4p forms are indicated on the right.





Right arrow Return to article