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Fig. 6. Immunofluorescent staining of Och1p-HA in the sec23-1 mutant. (A,B,E,F) Och1p-HA in sec23-1 (H1490) or (C,D) Och1p-HA plus cytochrome b(5)-opsin in sec23-1 (H1791) were grown at 24°C, followed by a 1 hour incubation with CHX at 37°C. The cells were fixed and immunostained with polyclonal antibody against HA (A,C,E). The cell samples on the right were viewed through Nomarski optics (B), or double stained with monoclonal antibody against opsin (D), or with DAPI (F). The arrows point to ER-like structures (C,D,E) or nuclei (F).