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Fig. 1. HA induces proteinase activity in 3LL cells. (A) HA induces gelatinolytic activity. 3LL cells were incubated with rooster comb HA or chondroitin sulfate A (CS-A) for 48 hours. Proteins in the conditioned medium were then TCA precipitated and used in a zymogram analysis to examine gelatinolytic activties in the conditioned medium. A major gelatinolytic band migrated close to 100 kDa and a minor band at 55 kDa. As a control, culture medium alone was also TCA precipitated and subjected to zymogram analysis (M). (B) 3LL cells were incubated with or without rooster comb HA for 24 hours. The cells were lysed in nonreducing SDS-PAGE sample buffer (lysate). Proteins in the conditioned medium were then TCA precipitated (CM). As a control, culture medium alone containing 0.5 mg ml^-1 HA was also TCA precipitated (M+). (C) HA enhances mRNA levels of several proteases. After treatment of 3LL cells with HA, cells were harvested at different times and poly(A)+ RNA was isolated. RNA (4 µg per sample) was analysed by northern blot for expression of the indicated genes.

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