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Fig. 2. Low-molecular-weight HA fragments induce MMP gene expression in MEFs and 3LL cells. (A) MEFs were treated or not with rooster comb HA of mixed molecular weight (R.C. HA), chondroitin sulphate A (CS-A), HealonTM high-molecular-weight HA (High HA), sonificated healon of intermediate molecular weight (Int HA) or with low-molecular-weight fragments of four to six residues (Low HA). Numbers refer to the final concentration of HA in the culture medium (mg ml-1). RT-PCR was performed to analyse the expression levels of Gapdh, Mmp-9 and Mmp-13. (B) 3LL cells were treated or not with 0.1 mg ml-1 Low HA. RT-PCR was performed to analyse the expression of Gapdh, Mmp-9 and Mmp-13. In one lane, cDNA was omitted (H2O control) as a negative control.





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