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Fig. 4. MAP kinase does not alter the distribution of mitochondria within axonal neuritis. Representative mitotracker-labelled images of NB2a/d1 cells treated for 2 hours with the MAP kinase inhibitor PD98059 or transfected 24 hours previously with constructs of constitutively active (Const-Act) or dominant-negative (Dom-Neg) MAP kinase are shown along with untreated controls. The accompanying graph presents densitometric analyses of the fluorescent intensity of mitotracker within the distal (Dist) compared to the proximal (Prox) half of axonal neurites, expressed as the percentage (mean±s.d.) within the distal neurite. Note that the distribution of mitotracker-labeled structures was not altered by the above treatments.





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