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Fig. 1. Structural organization of the ODF2 protein and subcellular localization of ODF2-GFP fusion proteins. (A) Scheme of ODF2 fusion constructs. As outlined, different parts of ODF2 were fused to GFP at its C-terminal end, resulting in a C-terminal GFP tag, or to GST at its N-terminal end. The leucine-zipper regions are shown as black boxes in the C-terminal region of ODF2. (B) Cytoplasmic versus nuclear distribution of ODF2-GFP fusion proteins expressed in cos-7 cells. Full length protein, ODF2NC, was slightly higher in the nucleus. The C-terminally truncated ODF2 proteins fused to GFP (ODF2NC1 and ODF2NC2) were mostly located outside of the nucleus, whereas the N-terminally truncated protein, ODF2N2C, fused to GFP was predominantly located inside the nucleus.
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