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Fig. 4. Self-interaction of ODF2 does not depend on the presence of the leucine zipper regions. ODF2-GST fusion proteins were incubated with ODF2-GFP fusion proteins, and GST proteins as well as associated proteins were isolated by affinity chromatography on glutathione-Sepharose beads. Proteins were separated using denaturing SDS-PAGE and probed with antibodies against GFP. The ODF2 constructs used for each assay are shown above with the first construct designating the GFP fusion detected by anti-GFP antibodies, and the second construct designating the GST fusion.





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