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Fig. 7. ODF2 does not bind directly to tubulin. Purified ODF2-GST fusion proteins were incubated with purified tubulin under conditions that promote microtubule formation. GST fusion proteins were isolated by affinity chromatography, separated using denaturing SDS-PAGE, transferred to Hybond-C, and probed with anti-ODF2 antibodies (A), and anti-{alpha}-tubulin antibodies (B). Whereas ODF2 fusion proteins were successfully isolated, {alpha}-tubulin was not coprecipitated.





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