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Fig. 5. Teratomas derived from reporter ES cells in adult male mice. At 4 weeks, ES cells injected in the testis capsule of syngenic mice (129 OLA) give rise to different tissues. Compare the normal testis tissue in A with a representative sample of ES-derived tissues in B, C and D. Bone (b), cartilage (ca), striated muscle (s), adipose tissue (a) and epithelial tissue (ep, red arrow) were found (B,C). ß-Galactosidase staining of BRE-lac1-derived teratomas showed staining in areas of cartilage and perichondrium (D, black arrowhead). Note that not all the chondrocytes show ß-galactosidase staining, suggesting regional differences in activation of Smad1/5 signaling in chondrocytes. Sections (7 µm) were stained either with Eosin/Hematoxilin (A-C) or Neutral Red and ß-galactosidase (D). Abbreviations: it, interstitial tissue; sp, spermatidia. Bar, 100 µm.





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