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Fig. 3. Intracellular localization of Cx33/Cx43 complex in Sertoli cells. (A-F) Cells were transfected with the vector containing Cx33-myc cDNA (D,E,F) or vector alone (A,B,C). In the absence of Cx33-myc, Cx43 was localized as linear punctuate staining at appositional plasma membranes between adjacent Sertoli cells (A, arrowhead). In the presence of Cx33-myc, Cx43 was solely detected in the perinuclear region (D, arrowhead), the myc signal was localized in the same region (E, arrowhead). Merging Cx33 and Cx43 micrographs revealed colocalization of two signals in Cx33-myc transfected Sertoli cells (F). Images are representative of three separate experiments. Magnification, x600.





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