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Fig. 4. Intracellular localization of Cx33/Cx43 complex in COS-7 cells. (A-C) In the absence of Cx33 or Cx33-myc, Cx43 staining was punctuated and linear at appositional plasma membranes between adjacent COS-7 cells and myc was undetectable. (D-F) In cells transfected with Cx33-myc cDNA the signals for myc (D) and Cx33 (E) colocalized similarly around the nucleus (F). (G-I) Cells were transfected with Cx33 or (J-L) Cx33-myc cDNAs in combination with Cx43. In the presence of Cx33 or Cx33-myc, Cx43 was solely detected in the perinuclear region (G,J). Merging Cx33 (or myc) and Cx43 micrographs revealed colocalization of the two signals in Cx33 or Cx33-myc transfected cells (I,L). Images are representative of three separate experiments. Magnification, x800.





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