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Fig. 6. Inhibition of gap junctional coupling by Cx33. (A-F) Phase-contrast and immunofluorescence photomicrographs (x300) of Sertoli cells transfected with vector containing Cx33-myc cDNA (D,E,F) or vector alone (A,B,C) microinjected with a mixture of Lucifer yellow and rhodamine dextran. No transfer of dye was observed in Sertoli cells overexpressing Cx33 (D-F). By contrast, Lucifer yellow was transferred to adjacent cells in the control (C), whereas rhodamine dextran was localized only within the microinjected cell (B). Microinjected cells are identified with arrows. The average number (mean±s.d., n=20) of communicating cells in control (vector alone) and Cx33-myc transfected Sertoli cells is given in the table. *P<0.001.





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