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Fig. 3. Actin mutation interferes with the TNF-induced death signal but not with TNF-induced NF-{kappa}B and p38 activation. (A) Wild-type and Actinmut cells were transiently transfected with the GFP expression vector and an expression vector of TNFR1(CD), TRADD, FADD, RIP or TRAF2. The death of the transfected cells (GFP positive) was calculated by counting sixteen random areas, and the differences in cell death were statistically significant (P<0.05, Student's t test). Three separate experiments were carried out with comparable results. (B) Wild-type and Actinmut cells were treated with TNF (100 ng/ml) for different time periods as indicated. I{kappa}B-{alpha} and phospho (P)-p38 levels were determined by Western blotting. Equal loading of total cell lyates is shown by p38 protein levels.





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