spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 4. Actin cleavage is not involved in TNF-induced L929 cell death. (A) L929 or HeLa cells were treated with TNF (100 ng/ml) or etoposide (Eto 40 µg/ml) for times as indicated. Actin and the 31 kDa fragment of actin were detected with the anti-actin polyclonal antibody against the C-terminal 11 residues. (B) Stable cell lines were generated from wild-type and Actinmut cells by transfection of an expression vector of flag-tagged 15 kDa ß-actin fragment (Wt+15K and Actinmut+15K). The expression of the 15 kDa fragment was detected with anti-flag antibody M2. (C) The sensitivity to TNF-induced cell death in Wt, Actinmut, Wt+15K and Actinmut+15K was measured with propidium iodide (PI) exclusion. Results represent the means±s.e. (n=3).





Right arrow Return to article