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Fig. 2. The wound-induced EF controlled wound-healing rates. (A) Circular lesions in rat cornea (3.5 mm in diameter at 0 hour) are labeled yellow with fluorescein and outlined with dots and shown at 10, 20 and 30 hours after wounding. Control (top row) healing is incomplete by 30 hours. Enhancing the wound-generated EF with PGE2 (second row), caused wound closure before 30 hours. Aminophylline, AgNO3 and ascorbic acid also enhanced wound-healing rates (not illustrated). Collapsing the EF with ouabain (bottom row) or furosemide (not shown) slowed wound healing which was incomplete by 30 hours. Neomycin also enhanced the wound-healing rate up to 30 hours after a wound (third row). (B) Corneal epithelial wound-healing rates varied with the wound-generated EF. Enhancing (with ascorbic acid, AgNO3, aminophylline and PGE2) or reducing (with ouabain and furosemide) the TCPD and therefore the steady wound-induced EF pharmacologically (x-axis), respectively enhanced and reduced the wound healing rate (µm/hour, mean±s.e.m. of 0-10 hours post wound). Regression formula for the correlation between TCPD and wound healing rate is y=34+0.12x-0.0002x2, Pearson correlation=0.91, correlation is significant at 0.01 level. A minimum of four experiments was performed in each group. Neomycin did not affect the TCPD, but enhanced the wound-healing rate significantly.
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