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Fig. 9. The number of new sprouts is a function of time and distance from the wound (EF strength). (A) At the wound edge (0-250 µm), new sprouts more than doubled in control corneas over the period from 16 hours (D) to 24 hours (E) (P<0.01), indicating that the EF effect was most potent at the leading edge between 16 and 24 hours. (B) At 500 µm-1 mm from the wound edge, there was no change in sprout number between 16 hour (F) and 24 hour (G) control values (P>0.05). Pharmacologically enhancing the endogenous EF, significantly increased nerve sprouts at 16 hours, for instance, compare the 16 hour control cornea (H) with the 16 hour aminophylline-treated cornea (I), (P<0.01), indicating that for all four drug-enhanced EFs the effects had penetrated to a depth of 1 mm between 0 and 16 hours. (C) At 250-500 µm from the wound edge, suppressing the endogenous EF with ouabain (K) or furosemide and preventing EF transduction with neomycin or tubocurare all significantly reduced nerve sprout numbers at 24 hours compared to 24 hour control values (J) (P<0.01), indicating that the EF effect had penetrated to a depth of at least 500 µm. Bars, 50 µm.
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