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Fig. 3. Effect of rTGF-ß1 on N-cadherin expression and the organization of N-cadherin-catenin-{alpha}-SMA complex in myofibroblasts. (A) Treatment of myofibroblasts with rTGF-ß1 for 7 days stimulates N-cadherin protein expression in a dose-dependent manner. Expression levels of ß-ctn and {alpha}-SMA proteins are not significantly changed. Tubulin protein expression is used as loading control. (B) Immunoprecipitation (IP) on lysates of myofibroblasts treated or not for 7 days with rTGF-ß1 (1 ng/ml) were performed to investigate the association of N-cadherin with ß-catenin and {alpha}-SMA. Total lysates were stained for N-cadherin and tubulin (loading control).





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