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Fig. 4. Effect of MAPK inhibitors on rTGF-ß1-stimulated N-cadherin expression and N-cadherin dependent myofibroblast invasion. (A) 4x10⁵ single myofibroblasts were seeded on a Matrigel coated filter, with rTGF-ß1 in the lower compartment to stimulate invasion. MAPK inhibitors were added in the upper compartment together with the myofibroblasts. Incubation was for 72 hours. Bars indicate the mean number of cells at the lower side of the filter (means of three results ± s.d.). The asterisk shows statistically significant inhibition of invasion. (B) Myofibroblast spheroids were cultured for 7 days in collagen gels in presence or not of rTGF-ß1 and MAPK inhibitors. Medium was exchanged on day 3 and 5. A representative micrograph for each condition is shown. Scale bars, 100 µm. (C) Western blot analysis of lysates from myofibroblasts treated for 7 days with rTGF-ß1 in presence or not of MAPK inhibitors. The upper part of the blot was probed for N-cadherin, the lower part for tubulin.

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