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Fig. 1. Identification of Gie as a novel subfamily of the small GTPases. (A) Comparison of amino-acid sequences of human Gie1, Gie2 and other small GTPase members. The amino-acid sequences were aligned using the ClustalW program. Amino-acid residues conserved among all the proteins are shaded in black, and those with 66-99% identity are in light gray. G1-G5 indicate well-conserved regions important for binding to phosphate/Mg²⁺ and the guanine base. The region considered to be a putative effecter domain is overlined. The C-terminal sequence for prenylation (CAAX box: A, aliphatic; X, any amino acid) and the N-terminal sequence for myristoylation (MG box) are boxed. The human sequence data are available from GenBank/EMBL/DDBJ under the accession numbers AK001564 (Gie1) and BC015408 (Gie2). (B) An unrooted tree constructed using ClustalW with standard parameters and viewed using TreeView. (C) Deduced amino-acid sequences from the indicated genes were aligned using the ClustalW. Amino-acid residues conserved among all the proteins are shaded in black, and those with 75-99% identity are in light gray. The accession numbers of Drosophila melanogaster CG7891 and Caenorhabditis elegans Y57G11C.13 are NP649769 and CAB16514, respectively, in the NCBI Entrez Protein database.

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