QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 4. Drosophila Gie is required for chromosome segregation. (A) Reduction of dGie mRNA levels by RNAi. Drosophila S2 cells were cultured with the control dsRNA (left) or dGie dsRNA (right) for 4 days, and the level of dGie transcript was measured by semiquantitative reverse-transcription PCR with decreasing amounts of the templates. Aurora-B mRNA was used as a loading control. (B) Flow cytometry analyses of the control (left) and dGie-dsRNA-treated (right) S2 cells. (C) The control (top) and dsRNA-treated (middle and bottom) S2 cells were stained with PicoGreen and the anti- ${alpha}$ -tubulin antibody to reveal DNA (green) and microtubules (red), respectively. Gie-depleted S2 cells displayed no separation of their chromatids before cytokinesis and remained with a thin chromatin string (arrowhead). They also had lagging chromosomes (arrows). Scale bar, 5 µm. (D, left) Representative immuno-stains of control (top) and dGie-dsRNA-treated S2 cells (bottom). The appearance of cells that had a chromatin string (arrowhead) or lagging chromosomes (arrows) at anaphase stage was measured. (D, right) The data are represented as percentages of means±s.e.m. from at least three independent experiments (each of 100 cells).

Return to article