QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 7. Co-localization and immunoprecipitation of Cx43 and P2X₇ proteins in ATP-s cells. (A) Cx43 immunolabeling, (B) P2X₇ immunolabeling and (C) TOPRO3 staining showing the nuclei of the J774 cells. Bar, 20 µm. (D) Reconstruction of 28 optical slices of 0.8 µm thickness. The green line indicates an orthogonal section from (D), projected laterally in (E); the red line indicates an orthogonal section from (D), projected laterally in (F); the blue line indicates the level of the 15th optical slice in (D). Confocal microscopy demonstrates co-localization of the Cx43 and P2X₇ proteins (yellow) to the membrane of the ATP-s cells (D-F, arrows). Notice the Cx43 (green) and P2X₇ (red) specific labeling located at membrane apposition areas (A,B, arrows). (G) Lane 1 shows a 69 kDa band corresponding to P2X₇ receptor obtained after immunoprecipitation of ATP-s cell lysates with Cx43 antibodies, and lane 2 shows the corresponding band obtained from whole ATP-s cell lysate after probing with the P2X₇ antibody_.

Return to article