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Fig. 1. (A) nNOS expression in parental SK-N-BE, clone-4 and pool of all the selected clone cells by immunoblot analysis. (B) Induction of nNOS expression in RA-treated parental SK-N-BE, clone-4 and pooled clone cells. The expression of nNOS was determined by western-blot analysis. Cells were exposed to 10 µM RA for a total of 10 days. 50 µg protein were subjected to SDS-PAGE and the blot was probed with anti-nNOS antibody. (C) Catalytic NOS activity evaluated through nitrite-plus-nitrate accumulation in the medium, expressed as a proportion of the activity in control cultures without RA (the absolute concentration of the control is ~1.5 µM). Bars are the mean±s.e.m. of three independent experiments performed in duplicate. *, P<0.01 (Bonferroni's test after ANOVA).





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