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Fig. 5. (A) N-Myc mRNA half-life. The intensity of the N-Myc mRNA signal was measured by laser densitometry of autoradiographs from northern blots and plotted as a function of time after exposure to actinomycin D. Parental and clone-4 cells treated with RA did not show alteration in the half-life of N-Myc mRNA. (B) Effect of RA treatment on luciferase activity in parental and clone-4 cells transfected with N-MycLuc and N-Myc{Delta}E2FLuc (3 µg). 12 hours after transfection, cells were exposed to RA for 24 hours. Luciferase activity is given as a percentage of the respective control condition in the absence of RA (hatched line) for each transfection condition. Bars are the mean±s.e.m. of four experiments. *, P<0.01 compared with control (Bonferroni's test after ANOVA). (C) DETA-NONOate dose-dependently decreased the activity of N-MycLuc reporter transiently transfected in parental cells. *, P<0.01 compared with control (Bonferroni's test after ANOVA).





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