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Fig. 1. Cdk5 plays a role in exocytosis in pituitary endocrine cells. Cultured rat anterior pituitary cells were pre-incubated with (+Ros) or without (-Ros) roscovitine (10 µM) for 4 hours and then incubated with the same medium for two collection periods of 30 minutes each (B1 and B2) followed by incubation in medium containing secretagog (2 mM BaCl₂, ST) with or without drug for 30 minutes. The media were collected and assayed for peptidylglycine ${alpha}$ -hydroxylating monooxygenase activity (PHM) (A). Extracts of rat anterior pituitary (AP), neurointermediate lobe (NIL), superior cervical ganglion (SCG) and cerebral cortex (CTX) were probed for Cdk5 (B, top) and p35 (B, bottom) by western blot. The same tissues were tested for p39 expression by RT-PCR (C, top). ß-actin amplification was carried out in parallel to provide a loading control (C, bottom). One representative experiment (of at least three) is shown.

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