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Fig. 4. Treatment with roscovitine alters granule localization beneath the plasma membrane. (A) Representative somatotropes from cultures treated with roscovitine as in Fig. 3 (- or +Ros) and then prepared for electron microscopy. Asterisks mark granules closely apposed to the plasma membrane; arrowheads mark granules separated from the plasma membrane; bar, 500 nm.(B) Granules were pooled based on their distance from the plasma membrane (top left) or from other granules (top right) in somatotropes treated with (filled bars) or without roscovitine (empty bars). Kolmogorov-Smirnov cumulative distributions for granule-to-membrane (bottom left) and granule-to-granule (bottom right) distances are shown. (C) Morphological identification of somatotropes in electron micrographs was confirmed by immuno-gold labeling of growth hormone; bar, 500 nm.
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