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Fig. 2. HERC5 mRNA expression in vascular cells. (A,B) Total cellular RNA from human ECs of different origin stimulated with either LPS or TNF{alpha} for the indicated periods of time was probed for HERC5 mRNA by northern blotting. HAEC were stimulated for 6 hours, FB and HSMC were stimulated for 9 hours. FB, human skin fibroblasts; HAEC, human aortic endothelial cells; HM2, a microvascular endothelial cell line; HSMC, human smooth muscle cells; HSMEC, human skin microvascular endothelial cells; HUMEC, human uterus microvascular endothelial cells; HUVEC, human umbilical vein endothelial cells. (C) HERC5 expression analysis by quantitative real-time RT-PCR. Cells were stimulated as described in A and quantitative real-time RT-PCR was performed. HERC5 expression levels are normalized to ß2-microglobulin expression. Changes in HERC5 mRNA were significant over time. (D) Comparison of HERC5 expression levels as determined by quantitative real-time RT-PCR in HSMECs, HUVECs and HSMCs.





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