|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 2. (A) Gyp5p and Gyl1p do not colocalize with actin. Yeast cells expressing Gyl1p-HA or Gyp5p-HA were stained by immunofluorescence and examined with a 3D deconvolution microscopy system. Images are projections of deconvoluted Z-series combining green, red and blue signals. Bars, 1 µm. First row: Gyl1p-HA is stained in red, actin is stained in green, DNA is stained in blue. Second row: Gyp5p-HA is stained in red, actin is stained in green, DNA is stained in blue. (B) Gyp5p-Myc and Gyl1p-HA localize within the septin ring. Yeast cells expressing Gyl1p-HA and Gyp5p-Myc were stained by immunofluorescence and examined with a 3D deconvolution microscopy system. Images are projections of deconvoluted Z-series combining green, red and blue signals. Cdc11p is stained in green, Gyl11p-HA is stained in red and Gyp5p-Myc is stained in blue. The magenta signal is the result of combination of red and blue signals. Bud emergence and post-cytokinesis stages are shown. Bars, 1 µm.
|
