JCS01362 Supplemental Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. Low magnification (103 objective) time-lapse movie of a control embryo starting at 10 hpf and ending at 12 hpf. PGCs were labeled with GFP by injection of GFP-nos1-3¢UTR RNA. Frames were captured every minute and replayed at 10 frames per second so that each second in the movie represents 10 minutes of real time.

• Movie 2 -

  Movie 2. low magnification (103 objective) time-lapse movie of PTX-nos1-3¢UTR injected embryo from 10 hpf to 12 hpf. PGCs were labeled with GFP by co-injection of GFP-nos1-3¢UTR RNA. Frames were captured every minute and replayed at 10 frames per second so that each second in the movie represents 10 minutes of real time.

• Movie 3 -

  Movie 3. high magnification (633 objective, digital zoom 2.3) confocal movie of a PGC at 13 hpf expressing PH^Crac-EGFP. Frames were captured every 10 seconds and replayed at a speed of 6 frames per second. One second in the movie corresponds to 1 minute of real time (10 minutes in total).

• Movie 4 -

  Movie 4. high magnification (633 objective) confocal movie of a migrating PGC labeled with PH^Crac-EGFP. Frames were captured every 5 seconds and replayed at a speed of 10 frames per second. One second in the movie corresponds to 50 seconds of real time (5 minutes in total).

• Movie 5 -

  Movie 5. high magnification (633 objective) time-lapse movie taken on a non-confocal light microscope of a PGC at 9 hpf expressing PH^Akt-EGFP. Frames were captured every 5 seconds and replayed at a speed of 10 frames per second. One second in the movie corresponds to 50 seconds of real time (5 minutes in total)

• Movie 6 -

  Movie 6. High magnification (633 objective, digital zoom 2.5) time-lapse confocal movie of a control PGC at 9 hpf. The membrane was labeled using farnesylated EGFP. Frames were captured every 10 seconds and replayed at a speed of 6 frames per second. One second in the movie corresponds to one minute of real time (10 minutes in total)

• Movie 7 -

  Movie 7. High magnification (633 objective, digital zoom 2.5) time-lapse confocal movie of a PGC expressing dnPI3K at 9hpf. The membrane was labeled using farnesylated EGFP. Frames were captured every 10 seconds and replayed at a speed of 6 frames per second. One second in the movie corresponds to one minute of real time (10 minutes in total).