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Fig. 4. The localization of PHCRAC-GFP to the membrane of PGCs is independent of SDF-1a/CXCR4b signaling. Localization of PHCRAC-GFP in (A) control PGCs, (B) cells expressing dnPI3K, (C) cells expressing CXCR4b morpholino (MO) or (E) PTX, as well as in PGCs in (D) an ody/ody mutant embryo. All cells also express the Vasa-GFP fusion protein, which labels the perinuclear granules. Arrows in A-E indicate the perinuclear granules.





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