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Fig. 2. Time course of Gq{alpha} translocation from the cell body to the rhabdomere. (A) Retinal cross-sections of dark-raised wild-type flies that were light exposed for 2.5 hours with a light intensity of ~50.7x103 lumens meter-2 and then incubated in the dark for 20 minutes or 150 minutes. Tissue sections were immunostained with an antibody against Gq{alpha}. (B) Quantitation of Gq{alpha} in the rhabdomeres from cross-sections of wild-type flies that were light-exposed for 2.5 hours and then incubated in the dark for 20 minutes, 60 minutes, 150 minutes or 240 minutes. Values are expressed as a percentage of Gq{alpha} signal in the rhabdomeres of the corresponding dark-raised flies. In the wild type, 89.3±1.76% of Gq{alpha} is localized to the rhabdomeres within 60 minutes of dark incubation. SEMs are indicated.





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