QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 5. (A) The translocation of G_q ${alpha}$ is independent of TRPL and Arrestin-2. Cross-sections from trpl³⁰² and arr2⁵ null mutants were stained with an antibody for G_q ${alpha}$ . All flies were either dark-raised or light exposed for 2 hours. G_q ${alpha}$ displayed normal, light-dependent translocation in both the trpl and the arr2 mutants, indicating their non-involvement in G_q ${alpha}$ 's translocation. (B) The translocation of TRPL and Arr2 is independent of G_q ${alpha}$ . Cross-sections from dark-raised and light-exposed dgq¹ mutants were immunostained for TRPL and Arr2. The dgq¹ mutant is a severe hypomorph that produces $~$ 1% of the wild-type levels of G_q ${alpha}$ (Scott et al., 1995). TRPL displays normal translocation, moving from the rhabdomere to the cell body upon illumination. Likewise, Arr2 displays normal light-dependent translocation from the cell body to the rhabdomere. These results indicate that the light-regulated translocation of both Arrestin2 and TRPL do not require G_q ${alpha}$ .

Return to article