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Fig. 6. The mechanism of Gq{alpha} translocation does not involve Shibere-mediated endocytosis. Dark-raised shiberets1 flies were first incubated at 30°C for 1-2 minutes in the dark to block endocytosis, as indicated by paralysis. Paralyzed shiberets1 flies continued to be incubated at 30°C but were either kept in the dark or transferred to the light for 1 hour. This incubation did not harm the flies because paralysis was reversible. Cross-sections were stained for Gq{alpha}. When endocytosis is disrupted, Gq{alpha} still displays light-regulated translocation from the rhabdomere to the cell body.





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