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Fig. 4. Effect of transfection with the Rab7 RNAi construct on Rab7 and Rab5 levels. HeLa cells were transiently transfected with empty pSUPER or pSUPER containing the Rab7 RNAi insert. Two parallel dishes were prepared for each construct. After 3 days the cells were prepared for western blotting with anti-Rab7 and anti-Rab5. Equal amounts of protein were loaded in each lane. Notice that Rab7 was heavily downregulated, whereas Rab5 was not affected.
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