|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 5. Effect of Rab7 RNAi on LC3 labelling. The cells were treated as in Fig. 4 and prepared for immunofluorescence with anti-LC3 (green) and anti-Lamp1 (red). Optical sections are shown. Arrowheads indicate large ring-like LC3-positive structures, which typically showed labelling for Lamp1 in the centre of the ring (arrowheads and inserts in B). These structures were only rarely seen in the Rab7 RNAi cells (C,D). Quantitation of the large LC3- and Lamp1-positive structures per cell is shown in E. Statistical significance was estimated using t-test. Bar, 10 µm.
|
