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Fig. 8. Late autophagic vacuoles were observed in cells expressing GFP-Rab7 T22N. HeLa cells were transfected with GFP-Rab7 T22N. One day later the cells were starved of amino acids for 2 hours and prepared for immunogold labelling with anti-GFP and 10 nm gold-coupled secondary antibodies. Cells expressing GFP-Rab7 T22N accumulated late autophagic vacuoles (AVd).





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