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Fig. 9. Recruitment of Rab7 to autophagic vacuoles was retarded in mouse fibroblasts deficient in Lamp1 and Lamp2. Control (A) and Lamp1/Lamp2-deficient (B) cells were transiently transfected with GFP-Rab7 (green), grown for 1 day, and starved of serum and amino acids (EBS) for 1 hour (A, B) or 2 hours (not shown). The cells were fixed and prepared for immunofluorescence staining with anti-LC3 (red). Optical sections are shown. Colocalisation of LC3 and Rab7 (arrowheads) was quantitated as the number of yellow dots per cell (C). The P values indicate statistical significance compared to the control cells (t-test). Bar, 20 µm.
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