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Fig. 4. Acceleration of motility by v-Src depends on PI 3-kinase, PLC and PLD. Rat-1/tsLA29 fibroblasts (A) and MDCK/tsLA31 cells (B) were pretreated with mitomycin C [except for `mitC(-)' group], and tested for wound healing during 6 hours in DMEM with 10% heat-inactivated FCS supplemented by the indicated concentrations of wortmannin, LY294002 (notice the different concentrations used for the two cell lines), NCDC, 1-butanol or 2-butanol. Values are means±s.e.m. of 20-50 microscopic fields for each condition, pooled from 2-5 experiments and expressed as the percentage of migration at 40°C over the group treated with mitomycin C alone (condition `0').





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