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Fig. 6. Growth factor binding isotherms at 4°C for 2 hours. (A) Rat-1/tsLA29 fibroblasts were incubated in suspension with the indicated final ¹²⁵I-PDGF concentrations; (B) ¹²⁵I EGF binding on MDCK/tsLA29 cells was performed in Petri dishes. Values are means±s.d. of specific binding in 6-9 dishes pooled from 3-4 experiments. Curves were drawn after non-linear fitting (r²>0.99), based on the hyperbola y=B_max*x/(K_d+x), where x is radioligand concentration, y is specific binding, B_max is a maximum specific binding and K_d is equilibrium dissociation constant. Graphs on the right result from Scatchard transformation.

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