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Fig. 4. Expression and localization of annexin II and {alpha}-catenin in melanoma cells. (A) Western blot analysis of parental and Tiam1-expressing melanoma cell lines. Lysates from parental and Tiam1-expressing 1F6 and MV3 cells were immunoblotted with annexin II and {alpha}-catenin antibodies. A smaller protein at 34 kDa is also visualized in MV3-Tiam1 cells (asterisk), which may be a proteolytical product of annexin II. ß-actin was used as an equal loading control. (B) Confocal images of melanoma cell lines stained for ALCAM (blue), annexin II (green) and F-actin (red). (C) Confocal images of melanoma cell lines stained for ALCAM (green), {alpha}-catenin (blue) and F-actin (red). Bars, 20 µm.





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