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Fig. 3. Mutation of NMTS alters the interphase Runx subnuclear organization. Point mutations within the Runx2 NMTS were generated using PCR-mediated mutagenesis. Deconvoluted images were analyzed of whole cells (HeLa) expressing either HA-tagged wild-type Runx2, an HA-tagged C-terminal deletion or one of the four HA-tagged NMTS point mutants. As shown, each of these mutants and wild-type Runx exhibits a punctate subnuclear distribution (top panel). Standardized mean subnuclear organization data for the indicated proteins are shown (right panel). A color map has been applied to the standardized values assigning red to higher values and green to lower values (see supplementary material). Using a repeated-measure analysis of variance (ANOVA) we detect significant differences at a 0.05 level in 17 of 25 parameters measured, as indicated by asterisks. Bar, 10 µm.





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